2017-8-11 16:0:32025-05-27 20:36:29
locus
BSU16950
BSU_16950
geneLength
1173
1176
ec
outlinks
bsu
BSU16950
BSU_16950
Gene
Coordinates
1,765,859 → 1,767,034
1,765,859 1,767,034
The protein
[SW|Localization]
extracellular (signal peptide) [Pubmed|18957862]
localizes during sporulation to both asymmetric septa and the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary]
extracellular (signal peptide) [Pubmed|18957862]
during vegetative growth: septal, low flourescence at periphery [pubmed|14731276]
during sporulation: both asymmetric septa and the prespore [pubmed|15758244]
Biological materials
Mutant
MGNA-B074 (pbpX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1073 NBRP B. subtilis, Japan]
MGNA-B074 (pbpX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1073 NBRP B. subtilis, Japan]
BKE16950 ([[gene|pbpX]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTTTTTCCTCCCAA, downstream forward: _UP4_TGATTGGCGATCTTCTTTTG
BKK16950 ([[gene|pbpX]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTTTTTCCTCCCAA, downstream forward: _UP4_TGATTGGCGATCTTCTTTTG
References
The protein
Protein family
[SW|beta-lactamase family] (according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
Structure
[PDB|3TG9] (a PBP from B. halodurans, aa 90-375, 28% identity)
Biological materials
GFP fusion
3107 [[gene|trpC]]2 [[gene|pbpX]]::pSG5047 (cat Pxyl-gfpa-[[gene|pbpX]]1-426) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]